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bmdms with imt1  (MedChemExpress)


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    MedChemExpress bmdms with imt1
    a , b Representative transmission electron microscopy (TEM) images ( a ) and cristae and mitochondrial aspect ratio (length/width) analysis ( b ) of non-stimulated (non-stim) and lipopolysaccharide (LPS)-stimulated wildtype (WT) and m.5019A > G BMDMs ( n = 3; LPS 6 h; mitochondria from a minimum of 9 cells were analysed per condition per biological replicate). Scale bars: 0.5 μm. Black arrows indicate mitochondria. c Mitochondrial (mt)DNA and mtRNA levels in cytosolic fraction of non-stim WT and m.5019A > G BMDMs ( n = 6). d , e Representative immunofluorescence staining of DNA and translocase of the outer membrane 20 (TOM20) coupled to confocal microscopy in LPS-stimulated WT and m.5019A > G BMDMs ( d ) and cytosolic DNA foci quantification in non-stim and LPS-stimulated WT and m.5019 A > G BMDMs ( e ) ( n = 3; LPS 24 h; minimum of 20 cells analysed per condition per biological replicate). White arrows indicate cytosolic DNA foci. f Ifnb1 expression (LPS 24 h) and interferon-β (IFN-β) release (LPS 6 h) in LPS-stimulated WT and m.5019 A > G macrophages pre-treated with cyclic GMP-AMP synthase (cGAS) inhibitor RU.521 or vehicle control (DMSO) for 1 h ( n = 3) ( P = 0.000262). g mt-Nd1 ( P = 0.000000008; P = 0.000946773), mt-Co3 ( P = 0.000000005; P = 0.000236469), Ifnb1 ( P = 0.000402; P = 0.000402) expression and IFN-β release in LPS-stimulated WT and m.5019A > G macrophages pre-treated with inhibitor of mitochondrial transcription 1 <t>(IMT1)</t> or vehicle control (DMSO) for 24 h ( n = 3; LPS 24 h). Data are mean ± s.e.m. n number represents independent biological replicates (mice) from a minimum of three independent experiments. P- values calculated using multiple two-tailed unpaired t tests corrected for multiple comparisons using the Benjamini, Krieger and Yekutieli method.
    Bmdms With Imt1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmdms+with+imt1/IMT1/pmc12635290-301-2-6
    Average 95 stars, based on 22 article reviews
    bmdms with imt1 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice"

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice

    Journal: Nature Communications

    doi: 10.1038/s41467-025-65023-4

    a , b Representative transmission electron microscopy (TEM) images ( a ) and cristae and mitochondrial aspect ratio (length/width) analysis ( b ) of non-stimulated (non-stim) and lipopolysaccharide (LPS)-stimulated wildtype (WT) and m.5019A > G BMDMs ( n = 3; LPS 6 h; mitochondria from a minimum of 9 cells were analysed per condition per biological replicate). Scale bars: 0.5 μm. Black arrows indicate mitochondria. c Mitochondrial (mt)DNA and mtRNA levels in cytosolic fraction of non-stim WT and m.5019A > G BMDMs ( n = 6). d , e Representative immunofluorescence staining of DNA and translocase of the outer membrane 20 (TOM20) coupled to confocal microscopy in LPS-stimulated WT and m.5019A > G BMDMs ( d ) and cytosolic DNA foci quantification in non-stim and LPS-stimulated WT and m.5019 A > G BMDMs ( e ) ( n = 3; LPS 24 h; minimum of 20 cells analysed per condition per biological replicate). White arrows indicate cytosolic DNA foci. f Ifnb1 expression (LPS 24 h) and interferon-β (IFN-β) release (LPS 6 h) in LPS-stimulated WT and m.5019 A > G macrophages pre-treated with cyclic GMP-AMP synthase (cGAS) inhibitor RU.521 or vehicle control (DMSO) for 1 h ( n = 3) ( P = 0.000262). g mt-Nd1 ( P = 0.000000008; P = 0.000946773), mt-Co3 ( P = 0.000000005; P = 0.000236469), Ifnb1 ( P = 0.000402; P = 0.000402) expression and IFN-β release in LPS-stimulated WT and m.5019A > G macrophages pre-treated with inhibitor of mitochondrial transcription 1 (IMT1) or vehicle control (DMSO) for 24 h ( n = 3; LPS 24 h). Data are mean ± s.e.m. n number represents independent biological replicates (mice) from a minimum of three independent experiments. P- values calculated using multiple two-tailed unpaired t tests corrected for multiple comparisons using the Benjamini, Krieger and Yekutieli method.
    Figure Legend Snippet: a , b Representative transmission electron microscopy (TEM) images ( a ) and cristae and mitochondrial aspect ratio (length/width) analysis ( b ) of non-stimulated (non-stim) and lipopolysaccharide (LPS)-stimulated wildtype (WT) and m.5019A > G BMDMs ( n = 3; LPS 6 h; mitochondria from a minimum of 9 cells were analysed per condition per biological replicate). Scale bars: 0.5 μm. Black arrows indicate mitochondria. c Mitochondrial (mt)DNA and mtRNA levels in cytosolic fraction of non-stim WT and m.5019A > G BMDMs ( n = 6). d , e Representative immunofluorescence staining of DNA and translocase of the outer membrane 20 (TOM20) coupled to confocal microscopy in LPS-stimulated WT and m.5019A > G BMDMs ( d ) and cytosolic DNA foci quantification in non-stim and LPS-stimulated WT and m.5019 A > G BMDMs ( e ) ( n = 3; LPS 24 h; minimum of 20 cells analysed per condition per biological replicate). White arrows indicate cytosolic DNA foci. f Ifnb1 expression (LPS 24 h) and interferon-β (IFN-β) release (LPS 6 h) in LPS-stimulated WT and m.5019 A > G macrophages pre-treated with cyclic GMP-AMP synthase (cGAS) inhibitor RU.521 or vehicle control (DMSO) for 1 h ( n = 3) ( P = 0.000262). g mt-Nd1 ( P = 0.000000008; P = 0.000946773), mt-Co3 ( P = 0.000000005; P = 0.000236469), Ifnb1 ( P = 0.000402; P = 0.000402) expression and IFN-β release in LPS-stimulated WT and m.5019A > G macrophages pre-treated with inhibitor of mitochondrial transcription 1 (IMT1) or vehicle control (DMSO) for 24 h ( n = 3; LPS 24 h). Data are mean ± s.e.m. n number represents independent biological replicates (mice) from a minimum of three independent experiments. P- values calculated using multiple two-tailed unpaired t tests corrected for multiple comparisons using the Benjamini, Krieger and Yekutieli method.

    Techniques Used: Transmission Assay, Electron Microscopy, Immunofluorescence, Staining, Membrane, Confocal Microscopy, Expressing, Control, Two Tailed Test

    Related Articles

    Transmission Assay:

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice
    Article Snippet: Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.. Pre-treatment of BMDMs with IMT1 (HY-134539; MedChemExpress; 10 μM) for 24 h or RU.521 (HY-114180; MedChemExpress; 10 μM) for 1 h prior to LPS stimulation were used to inhibit mitochondrial transcription and the cGAS-STING pathway, respectively.. ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.

    Electron Microscopy:

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice
    Article Snippet: Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.. Pre-treatment of BMDMs with IMT1 (HY-134539; MedChemExpress; 10 μM) for 24 h or RU.521 (HY-114180; MedChemExpress; 10 μM) for 1 h prior to LPS stimulation were used to inhibit mitochondrial transcription and the cGAS-STING pathway, respectively.. ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.

    Immunofluorescence:

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice
    Article Snippet: Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.. Pre-treatment of BMDMs with IMT1 (HY-134539; MedChemExpress; 10 μM) for 24 h or RU.521 (HY-114180; MedChemExpress; 10 μM) for 1 h prior to LPS stimulation were used to inhibit mitochondrial transcription and the cGAS-STING pathway, respectively.. ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.

    Staining:

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice
    Article Snippet: Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.. Pre-treatment of BMDMs with IMT1 (HY-134539; MedChemExpress; 10 μM) for 24 h or RU.521 (HY-114180; MedChemExpress; 10 μM) for 1 h prior to LPS stimulation were used to inhibit mitochondrial transcription and the cGAS-STING pathway, respectively.. ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.

    Membrane:

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice
    Article Snippet: Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.. Pre-treatment of BMDMs with IMT1 (HY-134539; MedChemExpress; 10 μM) for 24 h or RU.521 (HY-114180; MedChemExpress; 10 μM) for 1 h prior to LPS stimulation were used to inhibit mitochondrial transcription and the cGAS-STING pathway, respectively.. ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.

    Confocal Microscopy:

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice
    Article Snippet: Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.. Pre-treatment of BMDMs with IMT1 (HY-134539; MedChemExpress; 10 μM) for 24 h or RU.521 (HY-114180; MedChemExpress; 10 μM) for 1 h prior to LPS stimulation were used to inhibit mitochondrial transcription and the cGAS-STING pathway, respectively.. ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.

    Expressing:

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice
    Article Snippet: Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.. Pre-treatment of BMDMs with IMT1 (HY-134539; MedChemExpress; 10 μM) for 24 h or RU.521 (HY-114180; MedChemExpress; 10 μM) for 1 h prior to LPS stimulation were used to inhibit mitochondrial transcription and the cGAS-STING pathway, respectively.. ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.

    Control:

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice
    Article Snippet: Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.. Pre-treatment of BMDMs with IMT1 (HY-134539; MedChemExpress; 10 μM) for 24 h or RU.521 (HY-114180; MedChemExpress; 10 μM) for 1 h prior to LPS stimulation were used to inhibit mitochondrial transcription and the cGAS-STING pathway, respectively.. ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.

    Two Tailed Test:

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice
    Article Snippet: Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.Mouse IgG Isotype antibody (ThermoFisher Scientific; 20 μg/mL) was used as a control for anti-IFNAR antibody experiments.. Pre-treatment of BMDMs with IMT1 (HY-134539; MedChemExpress; 10 μM) for 24 h or RU.521 (HY-114180; MedChemExpress; 10 μM) for 1 h prior to LPS stimulation were used to inhibit mitochondrial transcription and the cGAS-STING pathway, respectively.. ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.ATP (Sigma; 5 mM) treatment of LPS-primed macrophages was used to activate the NLRP3 inflammasome, where indicated.



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    bmdms with imt1  (MedChemExpress)
    95
    MedChemExpress bmdms with imt1
    a , b Representative transmission electron microscopy (TEM) images ( a ) and cristae and mitochondrial aspect ratio (length/width) analysis ( b ) of non-stimulated (non-stim) and lipopolysaccharide (LPS)-stimulated wildtype (WT) and m.5019A > G BMDMs ( n = 3; LPS 6 h; mitochondria from a minimum of 9 cells were analysed per condition per biological replicate). Scale bars: 0.5 μm. Black arrows indicate mitochondria. c Mitochondrial (mt)DNA and mtRNA levels in cytosolic fraction of non-stim WT and m.5019A > G BMDMs ( n = 6). d , e Representative immunofluorescence staining of DNA and translocase of the outer membrane 20 (TOM20) coupled to confocal microscopy in LPS-stimulated WT and m.5019A > G BMDMs ( d ) and cytosolic DNA foci quantification in non-stim and LPS-stimulated WT and m.5019 A > G BMDMs ( e ) ( n = 3; LPS 24 h; minimum of 20 cells analysed per condition per biological replicate). White arrows indicate cytosolic DNA foci. f Ifnb1 expression (LPS 24 h) and interferon-β (IFN-β) release (LPS 6 h) in LPS-stimulated WT and m.5019 A > G macrophages pre-treated with cyclic GMP-AMP synthase (cGAS) inhibitor RU.521 or vehicle control (DMSO) for 1 h ( n = 3) ( P = 0.000262). g mt-Nd1 ( P = 0.000000008; P = 0.000946773), mt-Co3 ( P = 0.000000005; P = 0.000236469), Ifnb1 ( P = 0.000402; P = 0.000402) expression and IFN-β release in LPS-stimulated WT and m.5019A > G macrophages pre-treated with inhibitor of mitochondrial transcription 1 <t>(IMT1)</t> or vehicle control (DMSO) for 24 h ( n = 3; LPS 24 h). Data are mean ± s.e.m. n number represents independent biological replicates (mice) from a minimum of three independent experiments. P- values calculated using multiple two-tailed unpaired t tests corrected for multiple comparisons using the Benjamini, Krieger and Yekutieli method.
    Bmdms With Imt1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmdms+with+imt1/IMT1/pmc12635290-301-2-6
    Average 95 stars, based on 1 article reviews
    bmdms with imt1 - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    a , b Representative transmission electron microscopy (TEM) images ( a ) and cristae and mitochondrial aspect ratio (length/width) analysis ( b ) of non-stimulated (non-stim) and lipopolysaccharide (LPS)-stimulated wildtype (WT) and m.5019A > G BMDMs ( n = 3; LPS 6 h; mitochondria from a minimum of 9 cells were analysed per condition per biological replicate). Scale bars: 0.5 μm. Black arrows indicate mitochondria. c Mitochondrial (mt)DNA and mtRNA levels in cytosolic fraction of non-stim WT and m.5019A > G BMDMs ( n = 6). d , e Representative immunofluorescence staining of DNA and translocase of the outer membrane 20 (TOM20) coupled to confocal microscopy in LPS-stimulated WT and m.5019A > G BMDMs ( d ) and cytosolic DNA foci quantification in non-stim and LPS-stimulated WT and m.5019 A > G BMDMs ( e ) ( n = 3; LPS 24 h; minimum of 20 cells analysed per condition per biological replicate). White arrows indicate cytosolic DNA foci. f Ifnb1 expression (LPS 24 h) and interferon-β (IFN-β) release (LPS 6 h) in LPS-stimulated WT and m.5019 A > G macrophages pre-treated with cyclic GMP-AMP synthase (cGAS) inhibitor RU.521 or vehicle control (DMSO) for 1 h ( n = 3) ( P = 0.000262). g mt-Nd1 ( P = 0.000000008; P = 0.000946773), mt-Co3 ( P = 0.000000005; P = 0.000236469), Ifnb1 ( P = 0.000402; P = 0.000402) expression and IFN-β release in LPS-stimulated WT and m.5019A > G macrophages pre-treated with inhibitor of mitochondrial transcription 1 (IMT1) or vehicle control (DMSO) for 24 h ( n = 3; LPS 24 h). Data are mean ± s.e.m. n number represents independent biological replicates (mice) from a minimum of three independent experiments. P- values calculated using multiple two-tailed unpaired t tests corrected for multiple comparisons using the Benjamini, Krieger and Yekutieli method.

    Journal: Nature Communications

    Article Title: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice

    doi: 10.1038/s41467-025-65023-4

    Figure Lengend Snippet: a , b Representative transmission electron microscopy (TEM) images ( a ) and cristae and mitochondrial aspect ratio (length/width) analysis ( b ) of non-stimulated (non-stim) and lipopolysaccharide (LPS)-stimulated wildtype (WT) and m.5019A > G BMDMs ( n = 3; LPS 6 h; mitochondria from a minimum of 9 cells were analysed per condition per biological replicate). Scale bars: 0.5 μm. Black arrows indicate mitochondria. c Mitochondrial (mt)DNA and mtRNA levels in cytosolic fraction of non-stim WT and m.5019A > G BMDMs ( n = 6). d , e Representative immunofluorescence staining of DNA and translocase of the outer membrane 20 (TOM20) coupled to confocal microscopy in LPS-stimulated WT and m.5019A > G BMDMs ( d ) and cytosolic DNA foci quantification in non-stim and LPS-stimulated WT and m.5019 A > G BMDMs ( e ) ( n = 3; LPS 24 h; minimum of 20 cells analysed per condition per biological replicate). White arrows indicate cytosolic DNA foci. f Ifnb1 expression (LPS 24 h) and interferon-β (IFN-β) release (LPS 6 h) in LPS-stimulated WT and m.5019 A > G macrophages pre-treated with cyclic GMP-AMP synthase (cGAS) inhibitor RU.521 or vehicle control (DMSO) for 1 h ( n = 3) ( P = 0.000262). g mt-Nd1 ( P = 0.000000008; P = 0.000946773), mt-Co3 ( P = 0.000000005; P = 0.000236469), Ifnb1 ( P = 0.000402; P = 0.000402) expression and IFN-β release in LPS-stimulated WT and m.5019A > G macrophages pre-treated with inhibitor of mitochondrial transcription 1 (IMT1) or vehicle control (DMSO) for 24 h ( n = 3; LPS 24 h). Data are mean ± s.e.m. n number represents independent biological replicates (mice) from a minimum of three independent experiments. P- values calculated using multiple two-tailed unpaired t tests corrected for multiple comparisons using the Benjamini, Krieger and Yekutieli method.

    Article Snippet: Pre-treatment of BMDMs with IMT1 (HY-134539; MedChemExpress; 10 μM) for 24 h or RU.521 (HY-114180; MedChemExpress; 10 μM) for 1 h prior to LPS stimulation were used to inhibit mitochondrial transcription and the cGAS-STING pathway, respectively.

    Techniques: Transmission Assay, Electron Microscopy, Immunofluorescence, Staining, Membrane, Confocal Microscopy, Expressing, Control, Two Tailed Test